Nested PCR

chopstixn chopstixn at
Sat May 5 02:14:13 EST 2001

It might be good idea if you design the primers for second round of PCR with
higher Tm than ones for first PCR.  Especially when the 1st PCR's amplification
efficiency is not good enough, primers for 1st PCR are hanging around in the
reaction of 2nd PCR.

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