DNA size exclusion

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Wed May 9 03:33:22 EST 2001

In article <9d994t$ev8$1 at agate.berkeley.edu>, the eminent Louis Hom at
Univ. of California Berkeley Open Computing Facility wrote
>Does anyone know where I can find out what sizes of oligos will be
>excluded by the various sephadexes (G-75 and up)?  I would like to
>separate a ~100 bp dsDNA product from two 60-mer ssDNA oligos.

My guess is that you will not get good enough separation. Why not use a
small PAGE gel.

Homogeneous Fluorescent Reporting Systems for Real-Time Quantitative PCR: 

Optimisation, Probe Technology & Future Systems

4-5 September 2001
King Alfred's College, Winchester, UK 


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