sequence services

R. Jayakumar jakku at
Wed May 9 14:23:05 EST 2001

just send the PCR amplicon as such and they do the sequencing.  Probably
they clone it into a vector and use a universal primer or something.  But
most professional sequencing companies do PCR sequencing.  We normally clone
it into a T-tailed vector like pGEMT and then send it for sequencing.

----- Original Message -----
From: ""Deanne Bell"" <dbell at>
To: <methods at>
Sent: Wednesday, May 09, 2001 9:02 PM
Subject: Fw: sequence services

> Thanks to everyone for the sequencing references!!
> Now I have rookie question:
> I understand that sequencing can be done directly from a PCR product
> without cloning.
> Under what conditions should cloning be performed?
> I am now wondering if I really need to perform this step.
> Deanne Bell
> Molecular Markers Lab Technician
> USDA Agricultural Research Service
> 2021 S. Peach Ave.
> Fresno, CA 93727
> e-mail: dbell at
> phone: 559 453-3170
> fax: 559 453-3088
> ---


More information about the Methods mailing list