jakku at mrna.tn.nic.in
Wed May 9 14:23:05 EST 2001
just send the PCR amplicon as such and they do the sequencing. Probably
they clone it into a vector and use a universal primer or something. But
most professional sequencing companies do PCR sequencing. We normally clone
it into a T-tailed vector like pGEMT and then send it for sequencing.
----- Original Message -----
From: ""Deanne Bell"" <dbell at qnis.net>
To: <methods at hgmp.mrc.ac.uk>
Sent: Wednesday, May 09, 2001 9:02 PM
Subject: Fw: sequence services
> Thanks to everyone for the sequencing references!!
> Now I have rookie question:
> I understand that sequencing can be done directly from a PCR product
> without cloning.
> Under what conditions should cloning be performed?
> I am now wondering if I really need to perform this step.
> Deanne Bell
> Molecular Markers Lab Technician
> USDA Agricultural Research Service
> 2021 S. Peach Ave.
> Fresno, CA 93727
> e-mail: dbell at qnis.net
> phone: 559 453-3170
> fax: 559 453-3088
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