Anion Exchange Question

David Winterbourne sghk100 at
Thu May 10 03:31:06 EST 2001

Patrick Lynch wrote:

> ...  There's 20 mM phosphate in the eluted fraction as
> well - how much should this be diluted for application to the Mono-Q as you
> are not supposed to use negatively charged buffer ions with anion exchange?

The reason for this recommendation is that the buffer ion will bind to the
resin, and at an appropriate ionic strength be released from the resin,
modifying the buffering capacity in the liquid phase.

The chances are that this will have no effect on the results, as Patrick seems
to have found.

David Winterbourne
St. George's Hospital Medical School, London SW17 0RE, England

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