Fw: best way to store Mab-containing mouse ascites?

Marcin mnwoznia at med.uni-tuebingen.de
Thu May 10 05:08:20 EST 2001


> ----------
> : From: oswaldo <oswaldo at iats.csic.es>
> : I am not that familiar with Mabs and I have just received a couple ml.
> : ascitic fluid which I'll need to use for the next couple of years. Most
> : convenient way to store it would be liquid at 4C or 1:1 in glycerol at
> : -20, but I don't know whether this can affect its quality. Should I
> : aliquot and freeze it?. Any suggestions?. Thanks in advance,
> : 
> : Oswaldo
> : 
> 
> IMHO - dilute with glycerol and store at -20*C in small aliquotes.
> In my experience Mabs are pretty sensitive to a lot of freeze / thaw
> cycles.
> 
i keep them frozen in small aliquots, in -20 with 0.02% sodium azide, as i
had problems with glycerol solutions,

marcin

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