gst cross-linking problem

Rafael Maldonado rmaldonado at
Mon May 14 05:24:10 EST 2001

Ralf Weigel wrote:
> How can boiling in laemmli-buffer be
> less strong than Glycine/HCl treatment ?
> Any comments and suggestions are wellcome.

It does not suprise me. We have found some strong protein-protein
interactions resistant to laemli buffer, and even we can detect dimers
on standard SDS-PAGE gels.


Rafael Maldonado, Ph.D.
Division of Genetics
University of Alicante (Spain)

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