gst cross-linking problem

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon May 14 06:53:25 EST 2001


Aspartate decarboxylase tetramers are resistant to separation to monomers
in 2% SDS.  3min at 100 degrees for 70min is not enough.  10min is needed.
Mike.

On Mon, 14 May 2001, Rafael Maldonado wrote:

>
>
> Ralf Weigel wrote:
> >
> > How can boiling in laemmli-buffer be
> > less strong than Glycine/HCl treatment ?
> > Any comments and suggestions are wellcome.
> >
>
>
> It does not suprise me. We have found some strong protein-protein
> interactions resistant to laemli buffer, and even we can detect dimers
> on standard SDS-PAGE gels.
>
>
> -Rafa
>
>
> --
> Rafael Maldonado, Ph.D.
> Division of Genetics
> University of Alicante (Spain)
>




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