Connoisseurs of Ni NTA

Michael Witty mw132 at
Mon May 14 09:27:18 EST 2001

Here is one for connoisseurs of Ni-NTA.  When I loaded a protein (22kDa)
onto Ni-NTA, I eluted, roughly, 22, 44, 66kDa.  This protein has
disulphide bonds and I suspect some kind of bad breaking of disulphide
bonds and reforming between molecules.  Does anyone have similar
experience of this kind of thing?  Mike.

More information about the Methods mailing list