Very basic RT-PCR question
kaghoram at unity.ncsu.edu
Thu May 17 08:18:40 EST 2001
I am almost embarassed to ask this, but here I go anyhow: Are there any
known limits to the range of reverse transcriptase? One of my colleagues
is using a long-range thermo-polymerase to amplify long sequences and
build a library, but if the RT "fell off" before reaching the end of the
CDS, one is s**ewed, right?
I have never been able to get a straight answer to the limits-to-RT
question. Thanks in advance for any insight.
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