Very basic RT-PCR question

Karthik Aghoram kaghoram at unity.ncsu.edu
Thu May 17 08:18:40 EST 2001


I am almost embarassed to ask this, but here I go anyhow:  Are there any 
known limits to the range of reverse transcriptase?  One of my colleagues 
is using a long-range thermo-polymerase to amplify long sequences and 
build a library, but if the RT "fell off" before reaching the end of the 
CDS, one is s**ewed, right?  

I have never been able to get a straight answer to the limits-to-RT
question.  Thanks in advance for any insight. 




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