FPLC of 95% pure protein

Michael Witty mw132 at mole.bio.cam.ac.uk
Thu May 17 14:10:15 EST 2001


What about a 1ml, gravity flow column.  If you know the pI you might be
able to estimate in advance about how to choise between something like Q
or SP Sepharose.  Elute with steps of increaseing NaCl.  Mike.

 On Thu, 17 May 2001, David J. Meyer wrote:

> You don't give us much to go on, but you might consider hydroxyapatite. It
> sometime does very well for such purposes. Bio-Rad sells "disposable" (but
> actually very much reusable) "Econo-cartridges" which can easily be
> connected to FPLC. Be sure to read up on hydroxyapatite chromatography in
> Methods in Enzymology!
> --
> David J. Meyer, Ph.D.
> Pioneer Hi-Bred International, Inc.
> A DuPont Company
> 7300 NW 62nd Ave
> PO Box 1004
> Johnston, IA   50131-1004
>
> <Patrick Lynch> wrote in message
> news:20010517131536.21917.qmail at ww02.jatek.com...
> > Hi all,
> >
> > I have a protein that is approx. 90-95% pure but I want to give it a final
> > "polish" using an FPLC system.  What type of chromatography and column
> would
> > people recommend?  The protein is 90 kDa in size and I have about 1 mg of
> it.
> >
> > Cheers,
> >
> > Patrick
> >
> >
> > <http://www.biowww.net/forum/read.php?f=1&i=2106&t=2106>
> >
>
>
>




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