Very basic RT-PCR question

Stefi~ xxxgifalone at tin.it
Thu May 17 14:57:36 EST 2001


Karthik Aghoram <kaghoram at unity.ncsu.edu> wrote in message
9e0j3g$gmm$1 at uni00nw.unity.ncsu.edu...

> I am almost embarassed to ask this, but here I go anyhow:  Are there any
> known limits to the range of reverse transcriptase?  One of my colleagues
> is using a long-range thermo-polymerase to amplify long sequences and
> build a library, but if the RT "fell off" before reaching the end of the
> CDS, one is s**ewed, right?

I don't know if there are RTs for longer elongations but I think you could
try using a lower temperature (for example, 42°C instead of 48°C)...let me
know. Bye.
Stefano
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