Gateway cloning system by Invitrogen

Farida Safadi fsafadi at
Tue May 22 18:44:57 EST 2001

I have some experience with Gateway, Do you have specific questions.  I
asked the company which makes them (no affiliation) many questions, and
some of their tech assistants helped me better than the others. 

David Micklem wrote:
> In article <d45d9905.0105221145.7596f667 at>, April
> Shiflett <ashiflet at> wrote:
> >Hi,
> >Is there anyone who is using the Gateway recombinational cloning system for
> >cloning attB adapted PCR products using the "two-step" method?  I am having
> >some
> >difficulty trouble-shooting this protocol, and any advice would be appreciated.
> >Thanks!
> Would that be the "One tube" protocol?  I haven't tried it yet, but
> have been planning to, so I'd be interested to hear what
> problems/solutions you find!
> Best wishes,
> David
> --
> D.R. Micklem,              Time flies like an arrow...
> Dept. of Anatomy,          Fruit flies like a banana.
> Cambridge University,      Email:dmicklem at
> Cambridge, UK              Phone: +44 (1223) 333776
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