Gateway cloning system by Invitrogen

delios97 at hotmail.com delios97 at hotmail.com
Tue May 22 21:47:56 EST 2001


I have extensive experience with Life Technologies' Gateway, what's
the problem?



On 22 May 2001 12:45:37 -0700, ashiflet at arches.uga.edu (April
Shiflett) wrote:

>Hi, 
>Is there anyone who is using the Gateway recombinational cloning system for
>cloning attB adapted PCR products using the "two-step" method?  I am having some 
>difficulty trouble-shooting this protocol, and any advice would be appreciated.
>Thanks!





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