Gateway cloning system by Invitrogen

delios97 at delios97 at
Tue May 22 21:47:56 EST 2001

I have extensive experience with Life Technologies' Gateway, what's
the problem?

On 22 May 2001 12:45:37 -0700, ashiflet at (April
Shiflett) wrote:

>Is there anyone who is using the Gateway recombinational cloning system for
>cloning attB adapted PCR products using the "two-step" method?  I am having some 
>difficulty trouble-shooting this protocol, and any advice would be appreciated.

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