Fw: Stronger acrylamide gel.
wgallin at gpu.srv.ualberta.ca
Wed May 23 18:25:32 EST 2001
A further 2 cents.
The amount of bis-acrylamide will increase your gel strength; this is
the compound that cross-links the polyacrylamide polymers so it forms a
nice gel instead of a slab of phlegmy crap (yes I once left the bis
entirely out of my acrylamide mix). So, you might try increasing the
ratio of bis-acrylamide to acrylamide and see if it makes things
stronger. It should have only a minor effect on gel pore size.
Deanne Bell wrote:
> I will add my 2 cents worth to T. Fitzwater:
> Remember that ammonium persulfate and TEMED do not have long shelf lives,
> it is best to buy new stuff every once in awhile :-)
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