Two questions: Absolute EtOH and Carboxypeptidase Digestion
mw132 at mole.bio.cam.ac.uk
Mon May 28 08:00:51 EST 2001
> Hi, I know this is a molbio board, but I'm going to post my chem
> question anyways since I know there are a lot of helpful people on this board.
We are flattered! Though my chemistry is very weak.
> I recently performed an experiment involving the determination if an
> equilibrium constant in two solvent systems: (1) absolute ethanol and
> (2) water
What is your system? A peptidase reaction in water (buffer?) and ethanol
> would anyone know what %age the EtOH would be after absorbing water?
> (ie. ?95%?, 90%?)
No, not off hand but I think the keyword is azeotrope. Ethanol cannot be
made 100% by distillation and has a tendency to keep a certain amount of
water. Try checking a chemistry textbook for ethanol distillation (or
look at the data printed on the side of the ethanol bottle).
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