Two questions: Absolute EtOH and Carboxypeptidase Digestion
H.Veldman-2 at neuro.azu.nl
Mon May 28 09:20:38 EST 2001
Michael Witty schreef:
> > Hi, I know this is a molbio board, but I'm going to post my chem
> > question anyways since I know there are a lot of helpful people on this board.
> We are flattered! Though my chemistry is very weak.
> > I recently performed an experiment involving the determination if an
> > equilibrium constant in two solvent systems: (1) absolute ethanol and
> > (2) water
> What is your system? A peptidase reaction in water (buffer?) and ethanol
> (buffered ethanol?)
> > would anyone know what %age the EtOH would be after absorbing water?
> > (ie. ?95%?, 90%?)
> No, not off hand but I think the keyword is azeotrope. Ethanol cannot be
> made 100% by distillation and has a tendency to keep a certain amount of
> water. Try checking a chemistry textbook for ethanol distillation (or
> look at the data printed on the side of the ethanol bottle).
The azeotrope contains 95.57 % ethanol by weight (according to The Merck Index).
Laboratory for Experimental Neurology (NMZ)
University Medical Center Utrecht (AZU)
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