protein expression problem

Ricky Leung b883732 at logic.itsc.cuhk.edu.hk
Tue May 29 10:47:41 EST 2001


Hi Dr. Wolfgang,

: ... the insert obviously IS transcribed: Does it have the right 
: sequence i.e. is there no mutation/frameshift rendering it 
: indetectable?

I have done DNA sequencing and used High fidelity enzyme for any PCR
reactions in order to minimize any mutation or frameshifting due to
amplification error.  

Thank you

Ricky




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