Separation of multiple DNA fragments of 1.0-2 kb.

Zhongtang Yu zyu at interchange.ubc.ca
Tue May 29 14:57:19 EST 2001


Hello,

I want to separate multiple DNA fragments ranging from
1 to 2 kb. Because many fragments differ by less than 10 bp, I have not
been able to get a satisfactory separation by using 20 cm  polyacrylamide
gel (3.5% or 5%). Does any one have any trick to improve the
separation? Thank you.  

--
Zhongtang Yu, Ph.D.
Dept. of Animal Sciences, OSU
2027 Cofey Rd.
Colombus, OH 43210
     Tel: 614-292-3057
     Fax: 614-292-7116




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