IMMUNOBLOTTING - NEED HELP

L R lrzepiel at uoguelph.ca
Thu May 31 17:22:17 EST 2001


Hello,

I am getting a lot of unspecific Ab binding doing a western.  If I am
to use serum in the blocking buffer with the Ab, would it be the serum
of the organism the Ab was raised in or the opposite serum (ie. for
the primary Ab solution, the serum of the organism that the secondary
Ab was raised in and vice-versa)?

I greatly appreciate the help,

LR
lrzepiel at uoguelph.ca




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