plasmid wanted

Johannes Graumann graumann at sue.caltech.edu
Thu Nov 1 20:31:06 EST 2001


I found this thing from Clontech called pAtlas - seems perfect, but I bet 
they don't sell it aside from their array system. Will give them a call.

Joh

Johannes Graumann <graumann at blinky.caltech.edu> wrote:
> Hello,
> I'm looking for a plasmid cloning vector with the following properties:
> -NO multiple cloning site
> -just one (possibly obscure) restriction site resulting in blunt ends
> -as little overall restriction sites as possible

> Yep, got it right: I need a vector to subclone PCR products with primer 
> introduced restriction sites. Don't want to do TAing - strongly believe 
> in PfuTurbo.

> Any thoughts here what I might use?

> Thanks, Joh 


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