Fluo microscopy

Thierry Giffon tgiffon at stanford.edu
Thu Nov 1 20:20:30 EST 2001


Hi everybody,

I am facing a problem about fluorescence microscopy : I need to make a
double staining on T cells; intracellular (transcription factor) +
surface (bright), and for that I can use Alexa (FITC filter) and
Texas-Red (PI filter). Is there any reason to go for example T-R
intracell and ALEXA periph or the other way around ????

thanks a lot for your help

Thierry




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