krasel at wpxx02.toxi.uni-wuerzburg.de
Fri Nov 2 14:38:40 EST 2001
Johannes Graumann <graumann at blinky.caltech.edu> wrote:
> I'm looking for a plasmid cloning vector with the following properties:
> -NO multiple cloning site
> -just one (possibly obscure) restriction site resulting in blunt ends
> -as little overall restriction sites as possible
> Yep, got it right: I need a vector to subclone PCR products with primer
> introduced restriction sites. Don't want to do TAing - strongly believe
> in PfuTurbo.
> Any thoughts here what I might use?
You will be disappointed, but I am using good old Bluescript cut with
EcoRV for this case.
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: krasel at wpxx02.toxi.uni-wuerzburg.de */
/* "Science is the game we play with God to find out what His rules are." */
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