plasmid wanted

[Cornelius Krasel]

Johannes Graumann <graumann at blinky.caltech.edu> wrote:
> I'm looking for a plasmid cloning vector with the following properties:
> -NO multiple cloning site
> -just one (possibly obscure) restriction site resulting in blunt ends
> -as little overall restriction sites as possible
> 
> Yep, got it right: I need a vector to subclone PCR products with primer 
> introduced restriction sites. Don't want to do TAing - strongly believe 
> in PfuTurbo.
> 
> Any thoughts here what I might use?

You will be disappointed, but I am using good old Bluescript cut with
EcoRV for this case.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: krasel at wpxx02.toxi.uni-wuerzburg.de */
/* "Science is the game we play with God to find out what His rules are."  */