plasmid wanted

Johannes Graumann graumann at inky.caltech.edu
Sat Nov 3 17:31:45 EST 2001


> You could cut pBluescriptII with BssHII (which will remove the entire
> MCS) and then blunt with Klenow or T4 pol... That still won't get rid
> of all the sites in the rest of the vector though.

Exactly that was my backup plan - I will not fill in the ends though, but ligate 
in an oligo with a 8 nucleotide blunt end recognition side. Just hoped not to 
reinvent this ...

Joh
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