pre-running discont SDS gels???

[Lauri Lintott]

Wolfgang Schechinger wrote:
> 
> Hi all,
> 
> I noticed that some people pre-run discontinuous SDS gels (where stacking and
> separating gel have different pH in their respective buffers). According to
> Maniatis one should not do that because it will kill the pH difference and in
> my experience it is not necessary.
> 
> I don't want to make this experiment, so I better ask here.
> Any opinions?
> 
> Regards,
> Wo
> -----
> disclaimer: this message consists of
> randomly assembled ascii characters.
> a brain is reqired for decoding.
> -----
> Dr. Wolfgang Schechinger
> Institute of Biomedical Sciences
> Academia Sinica, Taipei, Taiwan R.O.C.
> e mail wolfsc at ibms dot sinica dot edu dot tw
> -----
> 
> ---

Your are right, they should not be prerun.

Lauri