pre-running discont SDS gels???
llintott at shaw.ca
Sat Nov 3 17:45:55 EST 2001
Wolfgang Schechinger wrote:
> Hi all,
> I noticed that some people pre-run discontinuous SDS gels (where stacking and
> separating gel have different pH in their respective buffers). According to
> Maniatis one should not do that because it will kill the pH difference and in
> my experience it is not necessary.
> I don't want to make this experiment, so I better ask here.
> Any opinions?
> disclaimer: this message consists of
> randomly assembled ascii characters.
> a brain is reqired for decoding.
> Dr. Wolfgang Schechinger
> Institute of Biomedical Sciences
> Academia Sinica, Taipei, Taiwan R.O.C.
> e mail wolfsc at ibms dot sinica dot edu dot tw
Your are right, they should not be prerun.
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