May glycerol interfere with Biorad protein assay?

Stefi~ xxxgifalone at tin.it
Mon Nov 5 14:04:55 EST 2001


Hi.
I have a protein sample (origin: peak eluted from chromatofocusing) that
contains glycerol. In my Biorad total protein assay (200uL Biorad+800uL
sample diluted) I obtain strange readings! With 2 uL of sample I obtain
A=0,300 but with 4 uL I read 0,200!!!! How can it be possibile? Does anyone
know what's happening? Thanx 2 every1!
Stefano
xxxgifalone at tin.it
(remove antispam xxx)




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