May glycerol interfere with Biorad protein assay?

[D.K.]

"Stefi~" <xxxgifalone at tin.it> wrote:
>Hi.
>I have a protein sample (origin: peak eluted from chromatofocusing) that
>contains glycerol. In my Biorad total protein assay (200uL Biorad+800uL
>sample diluted) I obtain strange readings! With 2 uL of sample I obtain
>A=0,300 but with 4 uL I read 0,200!!!! How can it be possibile? 

Ampholytes interfere with Bradford assay. Glycerol almost does not.

>Does anyone
>know what's happening? Thanx 2 every1!

Use BCA assay in this case and you will be fine.

DK