GST Contamination of GST-fusion proteins

Cornelius Krasel krasel at
Tue Nov 6 04:58:32 EST 2001

sridhar <sridhar.venk at> wrote:
> I am running into the same problem.
> do you have any suggestions?

You can try to purify away GST from your fusion protein by
traditional means of protein purification. If I recall correctly,
GST binds DEAE, so one possibility would be using a DEAE or Q
sepharose column.


/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: krasel at */
/* "Science is the game we play with God to find out what His rules are."  */

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