GST Contamination of GST-fusion proteins

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Tue Nov 6 04:58:32 EST 2001


sridhar <sridhar.venk at vai.org> wrote:
> I am running into the same problem.
> 
> do you have any suggestions?

You can try to purify away GST from your fusion protein by
traditional means of protein purification. If I recall correctly,
GST binds DEAE, so one possibility would be using a DEAE or Q
sepharose column.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: krasel at wpxx02.toxi.uni-wuerzburg.de */
/* "Science is the game we play with God to find out what His rules are."  */




More information about the Methods mailing list