DNA extraction mouse liver

barbro e johnsen b.e.johnsen at labmed.uio.no
Tue Nov 6 05:22:25 EST 2001


I have a problem with DNA extraction from mouse liver.  I use a standard
protocol, lysis w protK, phenol ext, precipitation w EtOH.  But when I
add EtOH, some white precipitate forms which is not my DNA.  I cannot
see any DNA threads as expected.  This protocol works fine for all other
mouse organs I have tried, exept liver.  What shall I do?  I have
checked various other protocols on the net, and they are more or less
the same as I am using.

B.




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