electroporation efficiency maximizing

Jon Warawa J.Warawa at bristol.ac.uk
Wed Nov 7 11:10:26 EST 2001


I am curious as to whether anyone has any ideas how to make high
competency electrocompetent cells.  I use DH10B's, and GibcoBRL claims
to get up to a magnitude of 10 to the 10 transformants per ug.  I get up
to 10 to the 9 on a good day, but am curious if there are any tricks
like special growth media which will push that number a bit higher.  I
know that slower growth at reduced temperature would be helpful, but any
other ideas?
Cheers,
Jon    (warawa at hotmail.com)




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