CSPD in westerns

[Jayakumar R]

hi..
  I am using CSPD as a substrate for AP conjugated antibodies in my western
blots.  I am getting a lot of backgrounds. Can anybody let me know any
protocol they are using for the same substrate which works very well.
mention what kind of blocking agent youa re using?
   thank you in advance
sincerely
jayakumar


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