Competent BL21 strains- preferred method anyone ?
Christopher W Kafer
ckafer at iastate.edu
Wed Nov 7 21:34:38 EST 2001
I also use the Inoue method or the single step method of Chung
(Current/short protocols). BL21 always transforms poorly. BL21 Gold
strains from Stratagene (?) give higher efficiencies. It's usually not a
problem to have lower efficiencies though since you dont use that strain
for cloning etc., no?
Moreland Gibbs <moreland.gibbs at mq.edu.au> wrote in
news:B80FFF5C.6F5%moreland.gibbs at mq.edu.au:
> I am trying to make competent BL-21 derived strains. Our usual preferred
> method for chemically competent cells is giving efficiencies of only
> 10^4-10^5/microgram DNA (Inoue method). I have also tried the method of
> Nishimura which also worked poorly. Next I will try electroporation but
> would like to ask...
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