who here still using phenol:chloroform:isoamyl alcohol?

Emanuela Guerra guerra at cmns.mnegri.it
Thu Nov 8 11:49:35 EST 2001

We still use organic extraction, and we still do one phenol extraction
followed by one separate chloroform:isoamyl alcohol extraction. For most
uses we saturate the phenol ourselves (also good training for our grad


Fun Science wrote:

> Who in this group still uses about a bottle of 500 mL of
> phenol:chloroform:isoamyl alcohol solution
> that is buffered -- every few months?
> Just curious to know what the percentage of
> labs using this product are.
> Cheers

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