lacIZ system for screening suppressors?

David Alexander dc.alexander at
Thu Nov 8 18:48:33 EST 2001

Hello All,

I would like to assess the suppressor activity of a gene I'm working with.
Literature from the 80's refers to a neat screen using panel of frameshifted 
and amber/ochre/opal mutants of lacI.
Does anyone still have and/or use this lacIZ system for screening suppressors?
Or is there a new fangled lux/luc/gfp system for doing the same thing?

Any advice and/or strains would be appreciated,



dc.alexander at


The Liu Lab
Department of Molecular and Medical Genetics
University of Toronto.
4382 Medical Sciences Building
1 King's College Circle.
Toronto, ON. Canada
M5S 1A8

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