competent cells advice?

Melanie Sohocki ms2241 at
Fri Nov 9 14:58:31 EST 2001

Hello All-
         I am having some insert instability problems with my subcloning. 
In the past, I have used the SURE cells from Stratagene to combat this 
problem, but both strains of these cells are resistant to kanamycin, the 
same marker on my plasmid. Unfortunately, I cannot simply change vector, 
because it is for a yeast-two hybrid application downstream. Any advice 
would be appreciated!



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