competent cells advice?
ms2241 at columbia.edu
Fri Nov 9 14:58:31 EST 2001
I am having some insert instability problems with my subcloning.
In the past, I have used the SURE cells from Stratagene to combat this
problem, but both strains of these cells are resistant to kanamycin, the
same marker on my plasmid. Unfortunately, I cannot simply change vector,
because it is for a yeast-two hybrid application downstream. Any advice
would be appreciated!
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