RT-PCR of low abundancy RNA

Mirek Svoboda svoboda at mou.cz
Sun Nov 11 13:27:26 EST 2001


Hello,
It depends on your substrate to be amplified: if you have G-rich, short  RNA
copies /100-2000nt/ AMV is better.
But if you have longer substrates with normal G-content, RevertAid, H
Minus-M-MULV RT from Fermentas /www.ferments.com/ is working very well
according to our experience. I heard that OmniScript from Quiagene is
superior, we will try it in next 14 days.
Best wishes,
Mirek 


<http://www.biowww.net/forum/read.php?f=1&i=4519&t=4375>




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