removal of nucleic acids.
Sergio
sergioal at bbm1.ucm.es
Mon Nov 12 08:24:17 EST 2001
RNAase + DNAase after lysis should work, but as recombinant proteins
purification usually involves a resin binding step (amylose, GST, metal, etc),
nucleic acids shouldn't be a big problem as they are washing out in this step.
Sergio
Itabajara da Silva Vaz Junior ha escrito:
> Does anybody knows which are the methods to remove nucleic acids
> prior recombinant protein purification?
> thanks__________________________________________________
> Itabajara da Silva Vaz Junior
> Disciplina de Imunologia Veterinária
> Faculdade de Veterinaria e
> Centro de Biotecnologia do RS
> Universidade Federal do Rio Grande do Sul
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