removal of nucleic acids.

Sergio sergioal at bbm1.ucm.es
Mon Nov 12 08:24:17 EST 2001


RNAase + DNAase after lysis should work, but as recombinant proteins
purification usually involves a resin binding step (amylose, GST, metal, etc),
nucleic acids shouldn't be a big problem as they are washing out in this step.
Sergio

Itabajara da Silva Vaz Junior ha escrito:

> Does anybody knows which are the methods to remove nucleic acids
> prior recombinant protein purification?
> thanks__________________________________________________
> Itabajara da Silva Vaz Junior
> Disciplina de Imunologia Veterinária
> Faculdade de Veterinaria e
> Centro de Biotecnologia do RS
> Universidade Federal do Rio Grande do Sul
> Av. Bento Goncalves 9500   C.P. 15005
> Predio 43421 - Campus do Vale
> 91501-970  Porto Alegre RS   Brasil
> Fone (51) 33166078    Fax (51)3316-73-09  [00 55 51 3316 7309]
> E-mail: ITA at DNA.CBIOT.UFRGS.BR
> http://www.ufrgs.br/depbiot/201/cbt201.htm
> http://www.ufrgs.br/favet/imunovet
>
> ---




More information about the Methods mailing list