RT-PCR of low abundancy RNA
bsssmt at bath.ac.uk
Tue Nov 13 05:59:32 EST 2001
could you let me know how the good the omniscript is?
Mirek Svoboda wrote:
> It depends on your substrate to be amplified: if you have G-rich, short RNA
> copies /100-2000nt/ AMV is better.
> But if you have longer substrates with normal G-content, RevertAid, H
> Minus-M-MULV RT from Fermentas /www.ferments.com/ is working very well
> according to our experience. I heard that OmniScript from Quiagene is
> superior, we will try it in next 14 days.
> Best wishes,
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