Digestion problems with EcoRI

Roland Hübner roland.hubner at ua.ac.be
Tue Nov 13 15:28:37 EST 2001


> > Hi Barbara
> > 
> > I don't know if this will help, but here is my 2 cents worth:
> > 
> > Lately, I have been having some concerns regarding genomic DNA 
> > methylation interfering with my EcoRI digests.  I have been 
> > looking for an isoschizomer, but have not been able to find anything.
> > 
> > Deanne Bell
> 
> 
> Hello,
> 
>  if you just need the 'right' overhang, Apo I might be worth a trial...
> 
> Greetings,
>  Roland

(addendum)

 Acs I or Xap I also cut GAATTC like Apo I ...

FWIIW,
Roland




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