Stable transformants using PCR amplified linear DNA
dk at no.email.thankstospam.net
Wed Nov 14 23:06:05 EST 2001
In article <3BF2F2FE.6A21FE8C at blood.ru>, Dusha <dusha at blood.ru> wrote:
>> And that makes sense. The flip side of the same coin: inclusion of
>> enzyme (say, BamH1) in the elecrtoporation mix was found to further
>> increase number of transformants.
>that sounds very interesting. could you give any references?
Insertional mutagenesis of Aspergillus fumigatus, Molecular & General
Genetics. 259(3):327-35, 1998
Co-transfer of restriction endonucleases and plasmid DNA into mammalian cells
by electroporation: effects on stable transformation. Mutation Research.
Additionally, about 10 years ago I discussed it with people who tried similar
things, but I don't know if it ever got published.
P.S. By the way, are you, by any chance, Dyusha Su*****ov? If yes, we used
to play ping-pong together and we have a bunch of common acquaintances.
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