SDS-PAGE problem

D.K. dk at
Mon Nov 19 21:27:50 EST 2001

jakku71 at (Jayakumar R) wrote:

>Which conditions are
>the best for separating two proteins which differ by hardly 1 kDa?

Take a regualar minigel in the PA range that is best suited for 
resolving proteins of the MW close to that of your protein, then 
run it 30-60 min beyond the point of dye eluting from the gel. 


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