SDS-PAGE problem

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Wed Nov 21 09:04:06 EST 2001


On Tue, 20 Nov 2001, R. John Lye wrote:

> Jayakumar R wrote:
>
> > The concentration of Bis in the gel is 1.6% (double the
> > normal of 0.8%). The Bis concentration is high since I need to use the gel
> > for seperating two proteins which differs by only 1 kDa.
>
> This might be a problem.  Its been a while since I looked at the charts
> of pore size vs bis concentration,but as I recall the pore size got larger
> as you went away from 0.8% in either direction
>
Smallest pore size occurs when the bis is 5% of the polyacrylamide
concentration (a 19:1 ratio).

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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