who here still using phenol:chloroform:isoamyl

Jonathan Rees jon at molbiol.net
Wed Nov 21 14:24:34 EST 2001

Buy your phenol-chloroform in from Sigma, they do it ready made with IAA at
your preferred pH (ie. 4.8 for RNA, 8 for DNA) - it comes in well sealed
glass jars, and if you keep it in the fridge its fine for a year, in my hands.

Jon Rees
Webmaster for 
http://molbiol.net/ - Portal for Bioinformatics and Molecular Biology


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