I reckon Amersham Pharamcia Biotech's GFX kit is the best I've ever used, with around 80% recovery...eluting in plain old hot water seems to improve recovery from all these kinds of kits. Jon Rees webmaster for http://molbiol.net - Portal for Bioinformatics <http://www.biowww.net/forum/read.php?f=1&i=4701&t=877>