microwaving glycerol--bad idea?

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Wed Nov 21 20:03:50 EST 2001


Dear Kyle, 

prepare a 50% stock in water and have it autoclaved (or filter sterilized).
Less viscosity, less hassle.

Enjoy, 
Wo

> 
> Hello, lately I have taken to heating glycerol in the microwave to make it
> easier to measure into solution (hot = less viscous), and I'd like to know
> whether this is a bad idea. Does the glycerol break down in the microwave?
> Lately I can't seem to bind His-tagged proteins to a nickel column, even though
> the resin bottle is relatively new and it has worked in the past (I use new
> resin each time, by the way). After thinking about it, the method of measuring
> out glycerol is the only change I can think of. Thanks for your help.
> 
> ... . . .  .  .  .    .    .    .     .     .     .      .      .      .
> legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com
> 
>    Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
>     moon musick:ritual:IDM:experimental(electronica):minimalism:glitch
> .      .      .     .     .     .    .    .    .   .   .   .  .  . . ...
> 
> 


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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw
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