microwaving glycerol--bad idea?

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Nov 21 20:18:13 EST 2001


Dear Tyson,
          how much ethylene aldehyde do you think I will get when I
autoclave my glycerol?  And will it decompose to acetate? (at a guess).
Regards, Mike.

On Wed, 21 Nov 2001, Tyson wrote:

> never tried heating it before...glycerol will start to decompose at its
> boiling point forming ethylene aldehyde, but it should be fine otherwise.
>
> ...but you should try going back to just pipetting it at RT just to see if
> it is the problem
> ...if your measuring small volumes, I usually just cut a bit off a 1ml
> pipette tip before pipetting dense solutions like glycerol. if you need
> large volumes it's better to weigh out the appropriate quantity
> (d=1.26g/ml).
>
> "Kyle Legate" <legatek at mcmail.cis.mcmaster.ca> wrote in message
> news:Pine.SOL.4.33.0111211547560.3171-100000 at mcmail.cis.mcmaster.ca...
> >
> > Hello, lately I have taken to heating glycerol in the microwave to make it
> > easier to measure into solution (hot = less viscous), and I'd like to know
> > whether this is a bad idea. Does the glycerol break down in the microwave?
> > Lately I can't seem to bind His-tagged proteins to a nickel column, even
> > though the resin bottle is relatively new and it has worked in the past (I
> > use new resin each time, by the way). After thinking about it, the method
> > of measuring out glycerol is the only change I can think of. Thanks for
> > your help.
> >
> > ... . . .  .  .  .    .    .    .     .     .     .      .      .      .
> > legatek at mcmaster.ca Kyle Legate            legatek at hotmail.com
> >
> >    Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
> >     moon musick:ritual:IDM:experimental(electronica):minimalism:glitch
> > .      .      .     .     .     .    .    .    .   .   .   .  .  . . ...
> >
>
>
>




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