RNA extraction

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Nov 21 23:44:54 EST 2001


In <20011121190046.28575.qmail at ww02.jatek.com>, A.Pappas wrote:
>
>To Whom it may concern,
>
>    Does anyone know of a way to extract RNA from tissue that is very high
>in protein content, ie. chondrasarcoma. I have been using Trizol but for
>this kind of tissue its kind of hard and I get no signal from a rt-pcr
>reaction, no ubiquitous cDNA signal... Any help would be appreciated.
>

Good old fashioned acidic guanidine thiocyanate extraction a la Chomchinski
(spelling completely wrong, no doubt, I think there are some "z's" in it) et al
with the phenol added separately works just fine for heart tissue; is that high
enough in protein?

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu

 




More information about the Methods mailing list