single strand dna migration

Wolfgang Schechinger wolfsc at
Fri Nov 23 03:58:12 EST 2001

Dear all, 

is there any way to "predict" migration behavior of ss dna on agarose gels?
we try to produce ss dna by s1 nuclease action on pcr product and get a faint 
band approx double (3.5kb) the size of the pcr product (1.8 kb). we see both 
bands in the same lane.

all innput is welcome!


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Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw


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