peptide antibody immobilization on a column

soenke behrends behrends at plexus.uke.uni-hamburg.de
Fri Nov 23 11:13:52 EST 2001


Subject: peptide antibody immobilization on a column

Dear netters, 

I would like to immobilize peptide antibodies (raised 
in rabbits) to a column for immunoaffinity purification 
of a protein. I would elute with the peptide used for 
immunization. 

Do you have a good working protocol or can you advise 
me on a material, kit, pre-packed column etc. from a 
specific supplier that works particularly well for 
that purpose?

Thanks for your time and help
Soenke






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