about restriction enzyme digestion

=?gb2312?q?shude=20yang?= sdyang68 at yahoo.com.cn
Sun Nov 25 22:50:30 EST 2001


Hello,

The pFL61 plasmid extracted from Ecoli showed three
bands when observed under UV in agar gel,While
digested pFL61 with BstXI showed only one band, its
size was larger than the smallest band of the
undigested pFL61,but smaller than the other two bands
of the undigested pFL61. My question are:1. Is BstXI
digestion  successful, 2.Why undigested pFL61 has a
band moving more fast than digested pFL61 plasmid?
Please tell me the reason.Thank you!

with best wishes
S.D.Yang

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