SV: query
Dag Rune Gjellesvik
dagrg at online.no
Tue Nov 20 10:06:57 EST 2001
Hi,
I am not sure what material the Centricon membrane is, but I've seen similar
problems. The solution was to shift to a "low-binding" PES-membrane. I think
several suppliers sell filters with this membrane type.
Dag Rune Gjellesvik
Biotec ASA,
Norway
"swamy" <gswamy at lamar.colostate.edu> skrev i melding
news:3BF9B036.7C0E71B at lamar.colostate.edu...
> I am trying to concentrate my protein(~23K) using centricon
> concentrators (MW-cut off 10,000 Da). I found that the protein is
> sticking to the membrane. Any alternative methods to concentrate
> proteins are welcome.
> Thanks in advance
>
> Swamy
>
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